Betekenis van:
elution
elution
Zelfstandig naamwoord
- the process of extracting one material from another by washing with a solvent to remove adsorbed material from an adsorbent (as in washing of loaded ion-exchange resins to remove captured ions); used to obtain uranium ions
Hyperoniemen
Voorbeeldzinnen
- Elution mode:
- Elution solvents (4.4)
- HPLC elution solvents
- Isocratic elution followed by gradient column cleaning:
- The rate of elution must not exceed 20 ml/min.
- Elution solvents must be prepared using HPLC-grade reagents.
- After elution of the sterol esters the chromatogram trace must not show any significant peaks (triglycerides).
- Elution buffers, prepared according to conditions for the analyser used (4.9).
- after 10 min. gradient elution during 30 min. to: A + B + C = 45 + 20 + 35 (V + V + V)
- The following conditions for isocratic elution are offered for guidance; other conditions may be used, provided they yield equivalent results (see also observations 9.1 and 9.2):
- It is important that the run time is sufficiently long for the elution of all the degradation products, otherwise late eluting peaks may interfere with subsequent chromatographic runs.
- The support material for the column elution method should be inert. Possible materials which can be employed are glass beads and sand.
- Leave the remaining methanol for 10 minutes to equilibrate with the resin and continue the elution at a rate not exceeding 20 ml/min. collecting the eluate in the same round-bottomed flask.