Betekenis van:
isothiocyanate

isothiocyanate
Zelfstandig naamwoord
    • a family of compounds derived from horseradish and radishes and onions and mustards; source of the hotness of those plants and preparations

    Hyperoniemen


    Voorbeeldzinnen

    1. Methyl isothiocyanate
    2. ALLYL ISOTHIOCYANATE
    3. Allyl isothiocyanate
    4. Standard solution: solution of allyl isothiocyanate in absolute alcohol containing 15 mg of allyl isothiocyanate per litre.
    5. Any allyl isothiocyanate present in the wine is collected by distillation and identified by gas chromatography.
    6. Using a filter for tetramethylrhodamine-5-isothiocyanate, Cy3-stained cells of R. solanacearum appear fluorescent red.
    7. Use of discs of pure paraffin impregnated with allyl isothiocyanate to create a sterile atmosphere
    8. No trace of allyl isothiocyanate must be present in the wine
    9. Check that the retention time of the peak obtained corresponds with that of the peak of allyl isothiocyanate.
    10. Using a filter for tetramethylrhodamine-5-isothiocyanate, Cy3-stained cells of C. m. subsp. sepedonicus appear fluorescent red.
    11. With the microsyringe, introduce a volume of the standard solution such that the peak corresponding to the allyl isothiocyanate can easily be identified on the gas chromatogram.
    12. Similarly introduce an aliquot of the distillate into the chromatograph. Check that the retention time of the peak obtained corresponds with that of the peak of allyl isothiocyanate.
    13. The titre is defined as the highest dilution at which optimum reaction occurs when testing a suspension containing 105 to 106 cells per ml of the homologous strain of R. solanacearum and using an appropriate fluorescein isothiocyanate (FITC) conjugate according to the manufacturer’s recommendations.
    14. Observe the slides immediately with a microscope fitted for epifluorescence microscopy at 630 or 1000x magnification under immersions oil. With a filter suitable for fluorescein isothiocyanate (FITC) eubacterial cells (including most gram negative cells) in the sample are stained fluorescent green.
    15. The titre is defined as the highest dilution at which optimum reaction occurs when testing a suspension containing 105 to 106 cells per ml of the homologous strain of C. m. subsp. sepedonicus and using an appropriate fluorescein isothiocyanate (FITC) conjugate according to the manufacturer's recommendations.