Betekenis van:
nucleotide

Voorbeeldzinnen

1. nucleotide sequencing analysis to allow determination of the deduced amino acid sequence at the cleavage site of the haemagglutinin molecule;
2. Viruses of H5/H7 subtype shall be subjected to the standard characterisation tests (nucleotide sequencing/IVPI) according to Council Directive 92/40/EEC.
3. Viruses of H5/H7 subtype shall be submitted without delay and shall be subjected to the standard characterisation tests (nucleotide sequencing/IVPI) according to that Diagnostic Manual.
4. Viruses of H5/H7 subtype shall be submitted without delay and shall be subjected to the standard characterisation tests (nucleotide sequencing/IVPI) according to Directive 92/40/EEC.
5. Viruses of H5/H7 subtype shall be submitted without delay and shall be subjected to the standard characterisation tests (nucleotide sequencing/IVPI) according to the said diagnostic manual.
6. Vaccine derived poliovirus (VDPV) (for the VDPV at least 85 % similarity with vaccine virus in the nucleotide sequences in the VP1 section)
7. Viruses of the H5/H7 subtype shall be submitted to the EURL without delay and shall be subjected to the standard characterisation tests (nucleotide sequencing/IVPI) according to the Diagnostic Manual.
8. National reference laboratories must also consider putting in place expertise and equipment that allow nucleotide sequencing of the haemagglutinin gene to determine whether or not there are multiple basic amino acids at the cleavage site of the haemagglutinin precursor protein for any LPAI H5 or H7 virus.
9. Conventional RT-PCR techniques on clinical specimens could, with the correctly defined primers, result in rapid detection and subtype (at least of H5 and H7) identification, plus a PCR amplicon product that could be used for nucleotide sequencing and have been demonstrated to have an important application by rapidly identifying subsequent outbreaks once the primary infected premises has been detected and the virus characterised.
10. For H5 and H7 viruses that do not have intravenous pathogenicity indices greater than 1,2, nucleotide sequencing of the haemagglutinin gene to determine whether or not there are multiple basic amino acids at the cleavage site of the haemagglutinin precursor protein must also be carried out immediately and the national reference laboratory and the competent authority in the country of origin must be informed as soon as the results are available so that control measures for HPAI can be fully implemented.