Betekenis van:
plasmin

plasmin
Zelfstandig naamwoord
    • an enzyme that dissolves the fibrin of blood clots

    Synoniemen

    Hyperoniemen

    Hyponiemen


    Voorbeeldzinnen

    1. Bovine plasmin (EC.
    2. Reagents for plasmin cleavage of caseins
    3. Plasmin cleavage of β-caseins to intensify γ-caseins
    4. Bovine plasmin (EC. 3.4.21.7), activity at least 5 U/ml
    5. Isoelectric focusing of plasmin-treated caseins in the presence of urea and staining of proteins
    6. Dissolving of the isolated caseins and submitting to plasmin (EC.3.4.21.7) cleavage
    7. Isoelectric focusing of plasmin-treated caseins from ewes’ and goats’ milk cheese containing different amounts of cows’ milk.
    8. Heat to 40 °C and add 10 μl plasmin (4.7.2), mix and incubate for one hour at 40 °C with continuous shaking.
    9. Note 2: If more sensitive silver staining (e.g. Silver Staining Kit, Protein, Pharmacia Biotech, Code No 17-1150-01) is preferred, plasmin-treated casein samples have to be diluted to 5 mg/ml.
    10. Addeo F., Moio L., Chianese L., Stingo C., Resmini P., Berner I, Krause I., Di Luccia A., Bocca A.: Use of plasmin to increase the sensitivity of the detection of bovine milk in ovine and/or caprine cheese by gel isoelectric focusing of γ2-caseins. Milchwissenschaft 45, 708-711 (1990).
    11. Addeo F., Moio L., Chianese L., Stingo C., Resmini P., Berner I, Krause I., Di Luccia A., Bocca A.: Use of plasmin to increase the sensitivity of the detection of bovine milk in ovine and/or caprine cheese by gel isoelectric focusing of γ2-caseins.
    12. Krause I., Berner I, Klostermeyer H.: Sensitive detection of cow milk in ewe and goat milk and cheese by carrier ampholyte — and carrier ampholyte/immobilized pH gradient — isoelectric focusing of γ-caseins using plasmin as signal amplifier. in: Electrophoresis-Forum 89 (B. J. Radola, ed.) pp 389-393, Bode-Verlag, München (1989).
    13. Heat to 40 °C and add 10 μl plasmin (4.7.2), mix and incubate for one hour at 40 °C with continuous shaking. To inhibit the enzyme add 20 μl ε-aminoproic acid solution (4.7.3), then add 200 mg of solid urea and 2 mg of dithiothreitol. Note: To obtain more symmetry in the focused casein bands it is advisable to freeze-dry the solution after adding the ε-aminocaproic acid and then dissolving the residues in 0,5 ml protein dissolving buffer (4.6).